Multiplex analysis of cytokines and chemokines in human serum was performed by using the 17 cytokine Bio-Plex ProHuman Cytokine Assay (BioRad) run on the Luminex 200 system according to the manufacturer's instructions with the exception that a proprietary BioRad assay dilution buffer was modified to contain reagents demonstrated to reduce the effects of heterophilic antibodies in multiplex immunoassays as previously described 21 (link). Data processing was performed by using Bio-Plex Manager 5.0, and analyte concentrations (in picograms per milliliter) were interpolated from standard curves.
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Multiplex Cytokine Analysis in Serum
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Other organizations : VA Palo Alto Health Care System, Stanford University, University of Mississippi Medical Center, Jackson Memorial Hospital, Omaha VA Medical Center, University of Nebraska Medical Center, Dallas VA Medical Center, George E. Wahlen Department of VA Medical Center, University of Utah, Georgetown University, Washington DC VA Medical Center
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Variable analysis
independent variables
- Multiplex analysis of cytokines and chemokines
- Concentrations of cytokines and chemokines (in picograms per milliliter)
- Proprietary BioRad assay dilution buffer modified to contain reagents demonstrated to reduce the effects of heterophilic antibodies in multiplex immunoassays
- Bio-Plex ProHuman Cytokine Assay (BioRad) run on the Luminex 200 system according to the manufacturer's instructions
- Positive controls: not explicitly mentioned
- Negative controls: not explicitly mentioned
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