Oxidative and Thermal Stress Assays

Stress resistance assays were performed as described previously^{20 (link)}, with modifications. Gravid adults were allowed to lay eggs for 12 hrs on NGM plates seeded with OP50. For the oxidative stress assay, L4-stage worms were transferred onto 5 μM FUDR-treated NGM plates with E. coli bacteria and 7.5 mM tert-butyl hydroperoxide (t-BOOH, Sigma, St. Louis, MO, USA) solution. For the thermotolerance assay, L4-stage worms were placed in a 35 °C incubator. The number of live worms was counted every 2 or 3 hr and recorded as dead when the worms did not respond to tactile stimuli with a platinum wire. All the assays were conducted at least twice independently. OASIS (https://sbi.postech.ac.kr/oasis/) and OASIS2 (https://sbi.postech.ac.kr/oasis2/) were used for statistical analysis^{67 (link),68 (link)}, and p values were calculated using a log-rank (Mantel–Cox method) test.

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Park H.E., Hwang W., Ham S., Kim E., Altintas O., Park S., Son H.G., Lee Y., Lee D., Heo W.D, & Lee S.J. (2021). A PTEN variant uncouples longevity from impaired fitness in Caenorhabditis elegans with reduced insulin/IGF-1 signaling. Nature Communications, 12, 5631.

Publication 2021

t-BOOH

Adults Assays Bacteria E coli Eggs Oasis Oxidative stress Platinum Tert butyl hydroperoxide Thermotolerance Worms

Corresponding Organization :

Other organizations : Korea Advanced Institute of Science and Technology, Pohang University of Science and Technology

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Variable analysis

independent variables

Oxidative stress: 7.5 mM tert-butyl hydroperoxide (t-BOOH)
Thermotolerance: 35 °C incubator

dependent variables

Number of live worms

control variables

Gravid adults were allowed to lay eggs for 12 hrs on NGM plates seeded with OP50
L4-stage worms were transferred onto 5 μM FUDR-treated NGM plates with E. coli bacteria

positive controls

None specified

negative controls

None specified

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