Thirteen HER2-positive breast cancer cell lines were used in this study (Table 1 ). AU565, BT474, HCC1419, HCC1569, HCC1954, HCC202, MDA-MB-453, and SKBR3 were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA), and EFM-192A and JIMT1 from the German Collection of Microorganisms and Cell Cultures (DSMZ; Braunschweig, Germany). ATCC and DSMZ authenticate human cell lines by DNA typing using short tandem repeats. SUM190PT and SUM225 were provided by S Ethier from Karmanos Cancer Institute in Michigan, USA, and KPL4 by J Kurebayashi from Kawasaki Medical School in Japan. The growth media are described in Table S1 . Cells were cultured for a maximum of 30 passages prior to use. ER statuses for the cell lines were obtained from the literature,15 (link)–19 (link) and only BT474 and EFM192A were ER-positive.
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HER2-positive Breast Cancer Cell Lines
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Corresponding Organization :
Other organizations : Cancer Genetics (United States), Oslo University Hospital, University of Oslo, Institute for Molecular Medicine Finland, University of Helsinki, VTT Technical Research Centre of Finland, UiT The Arctic University of Norway, Vestre Viken Hospital Trust
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Variable analysis
independent variables
- HER2-positive breast cancer cell lines
- Not explicitly mentioned
- Cell passages (maximum of 30 passages prior to use)
- ER status (only BT474 and EFM192A were ER-positive)
- Positive control: BT474 and EFM192A cell lines (ER-positive)
- Negative control: Not specified
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