Melanoma Cell Injection Protocol

Mouse melanoma cells B16F10 were grown as described [13 (link)] and extensively washed in phosphate buffered saline (PBS) prior to injection at 1.6 × 10⁵ cells/100 μL. B16F10-luc cells (mouse melanoma cells expressing luciferase) were kindly provided by Dr. Victoria Brentville (Scancell, Nottingham, UK) and injected at a dose of 4 × 10⁵ cells/100 μL. Cells were confirmed to be mycoplasma negative using the EZ-PCR Mycoplasma Test Kit (Biological Industries, Cromwell, CT, USA).

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Al-Rayahi I.A., Machado L.R, & Stover C.M. (2021). Properdin Is a Modulator of Tumour Immunity in a Syngeneic Mouse Melanoma Model. Medicina, 57(2), 85.

Publication 2021

EZ-PCR Mycoplasma Test Kit

Biological Cells Luciferase Melanoma Mouse Mycoplasma Phosphate Saline

Corresponding Organization : University of Northampton

Other organizations : Middle Technical University, University of Leicester

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Variable analysis

independent variables

Initial cell number of B16F10 cells injected (1.6 × 10^5 cells/100 μL)
Initial cell number of B16F10-luc cells injected (4 × 10^5 cells/100 μL)

dependent variables

Not explicitly mentioned

control variables

Cell culture conditions for B16F10 cells as described in the referenced publication [13]
Mycoplasma status of the cells (confirmed to be mycoplasma negative)

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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