Experiments involving animals complied with ARRIVE guidelines and were performed in accordance with the National Institutes of Health guide for the care and use of laboratory animals (NIH publication No. 8023, revised 1978) following protocols approved by the University of California, Davis, Institutional Animal Care and Use Committee. Adult male Sprague Dawley rats (225–250 g, 8–10 weeks old) were purchased from Charles River Laboratories and individually housed in standard plastic cages under controlled environmental conditions (22 ± 2 °C, 40–50% humidity) in fully accredited AALAC International facilities. Animals were housed in a 12 h light-dark cycle with access to food and water provided ad libitum. Animals were acutely intoxicated with DFP (n = 10) as previously described [43 (link)]. Vehicle (VEH, n = 15) controls were administered saline instead of DFP but were otherwise similarly treated. Experimental TBI was produced using a fluid percussion device (VCU Biomedical Engineering [44 (link)]) with the lateral orientation for impact [45 (link)] as previously published (n = 5 [46 (link)]). Sham controls (n = 5) underwent the same surgical procedures but were not administered the percussive impact. All animal and group characteristics for each data set can be found in Table 1.

Animal distribution and group characteristics for exploratory and validation data sets.

Table 1
Exploratory Data Set (Algorithm Development)Validation Data Sets
Detection MethodFluorescenceFluorescenceChromogen
Experimental ModelDFP IntoxicationDFP IntoxicationTBI
Brain Region AnalyzedDorsal Lateral ThalamusDorsal Lateral ThalamusHippocampus
Final Group SizeDFP vs. VEHTBI vs. ShamDFP:10VEH:15DFP:10VEH:11TBI:5Sham:5
Primary AntibodySourceRRIDaIBA-1Wako Laboratory ChemicalsAB_839504IBA-1Wako Laboratory ChemicalsAB_839504IBA-1Wako Laboratory ChemicalsAB_839504
CD68Bio-Rad LaboratoriesAB_2291300CD68Bio-Rad LaboratoriesAB_2291300

RRID: Research Resource Identification Number.

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