Experiments involving animals complied with ARRIVE guidelines and were performed in accordance with the National Institutes of Health guide for the care and use of laboratory animals (NIH publication No. 8023, revised 1978) following protocols approved by the University of California, Davis, Institutional Animal Care and Use Committee. Adult male Sprague Dawley rats (225–250 g, 8–10 weeks old) were purchased from Charles River Laboratories and individually housed in standard plastic cages under controlled environmental conditions (22 ± 2 °C, 40–50% humidity) in fully accredited AALAC International facilities. Animals were housed in a 12 h light-dark cycle with access to food and water provided ad libitum. Animals were acutely intoxicated with DFP (n = 10) as previously described [43 (link)]. Vehicle (VEH, n = 15) controls were administered saline instead of DFP but were otherwise similarly treated. Experimental TBI was produced using a fluid percussion device (VCU Biomedical Engineering [44 (link)]) with the lateral orientation for impact [45 (link)] as previously published (n = 5 [46 (link)]). Sham controls (n = 5) underwent the same surgical procedures but were not administered the percussive impact. All animal and group characteristics for each data set can be found in Table 1.
Animal distribution and group characteristics for exploratory and validation data sets.
Guignet M., Schmuck M., Harvey D.J., Nguyen D., Bruun D., Echeverri A., Gurkoff G, & Lein P.J. (2023). Novel image analysis tool for rapid screening of cell morphology in preclinical animal models of disease. Heliyon, 9(2), e13449.
Publication 2023
Adult Animal DeviceFoodHumidity Institutional animal care and use committee Laboratory animals Male Percussive River Saline Sprague dawley rats Surgical procedures
Corresponding Organization : University of California, Davis
Other organizations :
University of California, Irvine, Neurological Surgery
Microglial activation measured by IBA-1 and CD68 immunoreactivity in the dorsal lateral thalamus (for DFP intoxication) and hippocampus (for TBI)
control variables
Animal species: Adult male Sprague Dawley rats
Animal age: 8-10 weeks old
Animal weight: 225-250 g
Environmental conditions: 22 ± 2 °C, 40-50% humidity, 12 h light-dark cycle
Food and water access: ad libitum
controls
Positive control: DFP intoxication group
Negative control: Vehicle (VEH) group administered saline instead of DFP
Sham control group for TBI experiments (underwent surgical procedures but no percussive impact)
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