The detailed chromosome distribution information of each BnGR2R3-MYB genes was obtained from the ramie genome annotation documents. Circos (Krzywinski et al., 2009 (link)) was used to locate all the BnGR2R3-MYB genes on the ramie chromosome. Tandem, segmental duplication and collinearity within species were obtained by using Multiple Collinearity Scantoolkit (MCscanX) (Wang et al., 2012 (link)). Synteny blocks of the orthologous R2R3-MYB genes between ramie and other species were obtained also using MCscanX and both results visualized in TBtools (Chen et al., 2020 (link)). Non-synonymous and synonymous substitution of each duplicated BnGMYB genes were calculated using KaKs_Calculator 2.0 (Wang et al., 2010 (link)).
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