Quantitative Analysis of Neuronal Differentiation
Corresponding Organization :
Other organizations : VIB-UAntwerp Center for Molecular Neurology, University of Antwerp, University Hospital Cologne, University of Cologne, Scripps Research Institute, Florida Atlantic University, Medical University of Sofia, KU Leuven, VIB-KU Leuven Center for Brain & Disease Research, Brandeis University
Variable analysis
- Differentiation time (8 days)
- Total area covered with neurites and soma
- Ratio of skeletonized neurites per cell body area
- Distribution of primary and secondary neurite lengths
- Fraction of secondary neurites over the total
- Cell density (1 × 10^3 cells per well)
- Culture medium (B-27™ Plus neuronal culture system supplemented with retinoic acid, L-Glutamine, and penicillin/streptomycin)
- Medium refresh frequency (every other day)
- No positive or negative controls were explicitly mentioned.
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