Multiparameter Immune Cell Profiling

Commercial antibodies and staining reagents originated from BioLegend: CD45.1-FITC, CD45.2-APC, CD38-PE-Cy7, CD4-PerCP, CD8a-PerCP-Cy5.5, IFNAR1 biotin, and streptavidin conjugated to BV786; from BD Biosciences: CD16/CD32, CD138-BV650, B220-PacBlue, CD95 (APO-1/Fas)-PE; from ThermoFisher Scientific: GL7-A488, Ki67-efluor660 and viability dye fixable live/dead stain eFlour780. The 9D11 hybridoma expressing anti-idiotypic monoclonal IgG1 antibody, clone 9D11 (41 (link)), was kindly provided by Elisabeth Alicot, Boston Children’s Hospital, and was conjugated with iFluor647 succinimidyl ester (AAT Bioquest) in-house.

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Green K., Wittenborn T.R., Fahlquist-Hagert C., Terczynska-Dyla E., van Campen N., Jensen L., Reinert L., Hartmann R., Paludan S.R, & Degn S.E. (2021). B Cell Intrinsic STING Signaling Is Not Required for Autoreactive Germinal Center Participation. Frontiers in Immunology, 12, 782558.

Publication 2021

CD45.1-FITC CD45.2-APC CD38-PE-Cy7 CD4-PerCP CD8a-PerCP-Cy5.5 CD16/CD32

Anti idiotypic antibody Antibodies Biotin Cd138 Clone Cy5 5 Ester Fitc Hybridoma Igg1 Stain Streptavidin

Corresponding Organization : Aarhus University

Other organizations : Radboud University Nijmegen, Radboud University Medical Center

Top 5 similar protocols

Variable analysis

independent variables

CD45.1-FITC
CD45.2-APC
CD38-PE-Cy7
CD4-PerCP
CD8a-PerCP-Cy5.5
IFNAR1 biotin
Streptavidin conjugated to BV786
CD16/CD32
CD138-BV650
B220-PacBlue
CD95 (APO-1/Fas)-PE
GL7-A488
Ki67-efluor660
Viability dye fixable live/dead stain eFlour780
9D11 hybridoma expressing anti-idiotypic monoclonal IgG1 antibody, clone 9D11 (41 (link))

dependent variables

Measured outcomes

control variables

CD45.1-FITC
CD45.2-APC
CD38-PE-Cy7
CD4-PerCP
CD8a-PerCP-Cy5.5
IFNAR1 biotin
Streptavidin conjugated to BV786
CD16/CD32
CD138-BV650
B220-PacBlue
CD95 (APO-1/Fas)-PE
GL7-A488
Ki67-efluor660
Viability dye fixable live/dead stain eFlour780
9D11 hybridoma expressing anti-idiotypic monoclonal IgG1 antibody, clone 9D11 (41 (link))

positive controls

9D11 hybridoma expressing anti-idiotypic monoclonal IgG1 antibody, clone 9D11 (41 (link))

Annotations

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