Antibody Characterization Protocol

Antibody concentration, reactivity against specific antigens, and indirect immunofluorescence were as described (1 (link)). M55 was used as a positive control in all polyreactivity ELISAs (22 (link)).

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Meffre E., Schaefer A., Wardemann H., Wilson P., Davis E, & Nussenzweig M.C. (2004). Surrogate Light Chain Expressing Human Peripheral B Cells Produce Self-reactive Antibodies. The Journal of Experimental Medicine, 199(1), 145-150.

Publication 2004

Antibody Antigens Elisas Indirect immunofluorescence

Corresponding Organization :

Other organizations : Hospital for Special Surgery, Rockefeller University, Cornell University, Oklahoma Medical Research Foundation, National Institutes of Health, National Cancer Institute, Howard Hughes Medical Institute

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Protocol cited in 21 other protocols

Variable analysis

independent variables

Antibody concentration

dependent variables

Reactivity against specific antigens
Indirect immunofluorescence

control variables

Not explicitly mentioned

controls

Positive, M55

Annotations

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