Infarction volumes were quantified on Nissl-stained sections using the “indirect” morphometric method. Immunohistochemistry was performed as described before6 (link). To assess microvessel remodeling, double-labeling of anti-type IV Collagen (1:10, SouthernBiotech) with anti-Ki67 (1:500, Abcam) (a general cell proliferation marker) was pursued as a surrogate marker of angiogenic-related events. To study neurogenic-related events, we double-stained anti-DCX (1:100, Abcam) with anti BrdU (1:50, Invitrogen) as a surrogate marker of neurogenesis.
To clarify that Ki67 positive cells are not proliferating microglia/macrophages we double-stained Ki67and Iba1.
To clarify that Ki67 positive cells are not proliferating microglia/macrophages we double-stained Ki67and Iba1.
