mIMCD-3 (mouse inner medulla collecting duct) mouse cell lines were purchased from ATCC.
RMC219-Tet-inducible SMARCB1 cell line: The tetracycline-inducible pIND20-fSNF5-HA vector (35 (link)) was kindly donated by Bernard E. Weissman. The pInducer20 empty backbone (36 (link)) was a gift from Stephen Elledge (Addgene plasmid # 44012). Lentivirus was generated in HEK-293T cells and used to generate stable tet-inducible cell lines as previously described (37 (link)). RMC219 (also called JHRCC219), a human tumor cell line that was established in a previous study (23 (link)), was infected with tetracycline-inducible pIND20-fSNF5-HA vector. Cells were treated with 2 μg DOX for 3 d to reconstitute SMARCB1 (SNF5).
MSRT1 (Melinda Soeung renal tumor 1) was generated using CRISPR-Cas9 gene editing technology as described inSI Appendix, Figs. S2 and S3 and Supporting methods .
RMC219-Tet-inducible SMARCB1 cell line: The tetracycline-inducible pIND20-fSNF5-HA vector (35 (link)) was kindly donated by Bernard E. Weissman. The pInducer20 empty backbone (36 (link)) was a gift from Stephen Elledge (Addgene plasmid # 44012). Lentivirus was generated in HEK-293T cells and used to generate stable tet-inducible cell lines as previously described (37 (link)). RMC219 (also called JHRCC219), a human tumor cell line that was established in a previous study (23 (link)), was infected with tetracycline-inducible pIND20-fSNF5-HA vector. Cells were treated with 2 μg DOX for 3 d to reconstitute SMARCB1 (SNF5).
MSRT1 (Melinda Soeung renal tumor 1) was generated using CRISPR-Cas9 gene editing technology as described in
