An RNA-Seq library was produced for C. americanus from total RNA extracted from four pooled larvae with the RNeasy Mini Kit (Qiagen) and subsequently treated with RQ1 DNase I (Promega). Ribosomal RNA was reduced with the RiboZero Gold rRNA removal kit (Epicentre). The library was generated with the RNA ScriptSeq v2 RNA-Seq library preparation kit (Epicentre) and quantified with the Agilent 2100 Bioanalyzer RNA 6000 Pico Chip. RNA sequencing was carried out at the University at Buffalo Next Generation sequencing facility using Rapid 150-cycle paired-end sequencing on a single Illumina HiSeq 2000 lane. Due to technical error, only single end reads were obtained (approximately 33 million 150 bp reads). CLC Genomics Workbench (http://www.qiagenbioinformatics.com) was used for de novo sequence assembly with default parameters, which allowed the software to automatically select kmer size, bubble size, and paired distances. Assembled contigs were grouped into a custom sequence database in Geneious R7.1 (Biomatters, http://www.geneious.com) (Kearse et al. 2012 (link)) and queried with phasmavirus amino acid sequences using the Basic Local Alignment Search Tool (BLAST) (Altschul et al. 1990 ) algorithm tBLASTn and an expect value cutoff of 10−5.