The Puro-PLA procedure was performed as previously described (18 (link)). Briefly, after puromycylation with 3 μM puromycin for 15 min, neurons were fixed with 4% paraformaldehyde (PFA)–sucrose for 20 min at room temperature (RT). After fixation, cells were permeabilized with 0.5% Triton X-100 in phosphate-buffered saline (PBS) for 15 min, blocked in 10% FBS in PBS for 30 min, and incubated with primary antibody pairs diluted in blocking buffer for 1.5 hours at RT. PLA probes (Duolink In Situ PLA Probes, Sigma-Aldrich) were used according to the manufacturer’s instructions. Ligation and amplification reactions were performed according to the manufacturer’s recommendations (Duolink In Situ Detection Reagents Red, Sigma-Aldrich). After amplifications, cells were postfixed in 4% PFA-sucrose for 10 min at RT for better signal stability, processed further for immunocytochemistry with Hoechst staining for nuclei visualization, and mounted with fluorescent mounting medium (Dako).