Cases were a convenience sample of naturally occurring mortalities on 6
large-scale commercial beef feedlots in Alberta and Saskatchewan, Canada, from
January 2018 to January 2022. Based on gross and histopathological diagnoses,
the study included 18 cases of BIP, 24 cases of BP, and 13 cases of AIP. From
each case, lung samples were tested as follows: fresh tissue for routine
bacterial culture and mycoplasmal culture (cranioventral and caudodorsal lung,
separately), fresh tissue for polymerase chain reaction (PCR) testing for
viruses (caudodorsal lung only), and formalin fixation for histopathology
(cranioventral and caudodorsal lung, separately). Clotted blood was collected
from the heart, and the serum was used for cytokine analysis. Bronchoalveolar
lavage fluid was harvested from 13 cases for surfactant analysis (Supplemental Table S1 ).
large-scale commercial beef feedlots in Alberta and Saskatchewan, Canada, from
January 2018 to January 2022. Based on gross and histopathological diagnoses,
the study included 18 cases of BIP, 24 cases of BP, and 13 cases of AIP. From
each case, lung samples were tested as follows: fresh tissue for routine
bacterial culture and mycoplasmal culture (cranioventral and caudodorsal lung,
separately), fresh tissue for polymerase chain reaction (PCR) testing for
viruses (caudodorsal lung only), and formalin fixation for histopathology
(cranioventral and caudodorsal lung, separately). Clotted blood was collected
from the heart, and the serum was used for cytokine analysis. Bronchoalveolar
lavage fluid was harvested from 13 cases for surfactant analysis (
