Trp2, AH1 and WT1 peptide libraries and peptides were synthesized by GenScript. CoPoP was synthesized as previously reported (32 (link)). The following lipids were used to produce liposomes: dioleoylphosphatidylcholine (DOPC; Corden #LP-R4–070), cholesterol (PhytoChol; Wilshire), and synthetic PHAD-3D6A (Avanti; #699855). QS-21 was purchased from Desert King (part number NC0949192). The APC-CD8a (Clone: CT-CD8a) antibody was obtained from Accurate Chemical and Scientific Corporation (#ACL168APC). The following antibodies were obtained from BioLegend: APC-CD8a (Clone: CT-CD8a; #100712), FITC-CD4 (Clone: GK1.5; #100405), AF700-CD45 (Clone: 30-F11; #103127), APC-Cy7-CD44 (Clone: IM7; #103027), PE/Cy7-CD62L (Clone: MEL-14; #104417), PerCP-Cy5.5-PD-1 (Clone: RMP1–30; #109119), pacific blue-IFN-γ (Clone: XMG1.2; #505818), BV605-TNF-α (Clone: MP6-XT22; #506329), PE/Cy7-Granzyme B (Clone: QA16A02; #372213). Anti-mouse PD-1 (#BP0146) and anti-mouse CTLA-4 (#BP0131) were acquired from Bio X Cell. Other reagents used were Golgiplug/Brefeldin A (BD; #555029), Live/Dead dye (Invitrogen; #L34857), Fc-block (Clone: 2.4G2; BD #553142), fixation/permeabilization kit (BD #554714), red blood cell (RBC) lysis buffer (BioVision #5830), Collagenase Type I (Gibco #17018–029), and DNase I (Roche #04536282001).