Multimodal Imaging of Tau and Amyloid

¹⁸F-MK-6240 and ¹¹C-Pittsburgh Compound B (PiB) were used to quantify aggregated tau and cortical β-amyloid, respectively, using previously published methods [46 (link), 47 (link)]. PET data were collected using either a Siemens Biograph Horizon PET/CT or Siemens EXACT HR+ tomograph. Dynamic data were acquired for 20 min (5 min × 4 frames) following a 70-min uptake period for MK-6240 and 0–70 min (2 min × 5 frames, 5 min × 12 frames) beginning with tracer injection for PiB. T1-weighted magnetic resonance imaging (MRI) was performed to delineate anatomical regions. MRI and PET image processing and quality control were performed using a pipeline that uses MATLAB (The Mathworks, Inc., Natick, MA) and SPM12 (University College London). Details regarding radioligand synthesis, image acquisition, processing, and analysis of MRI, MK-6240 PET images [46 (link)], and PiB PET images [47 (link)] have been previously described.