OFs were lysed and processed as described previously.20 (link) Antibodies targeting p-AMPKα (rabbit anti-p-AMPKα), AMPKα (rabbit anti-AMPKα), p-AMPKβ (rabbit anti-p-AMPKβ), AMPKβ1/2 (rabbit anti-AMPKβ1/2), p-ACC (rabbit anti-p-ACC), ACC (rabbit anti-ACC), and β-tubulin (rabbit anti-β-tubulin) were all obtained from Cell Signaling Technology (Danvers, MA, USA), and AMPKβ (mouse anti-AMPKβ) was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Protocol detail
AMPK Signaling Pathway Characterization
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Corresponding Organization : University of Rochester
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Variable analysis
independent variables
- Lysis and processing of OFs as described previously
- P-AMPKα
- AMPKα
- P-AMPKβ
- AMPKβ1/2
- P-ACC
- β-tubulin
- AMPKβ
- Samples were processed as described in the referenced study (link provided)
- No positive or negative controls were explicitly mentioned in the provided information.
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