Three pools of leaves, each of which contained five control or stressed plants of the two studied genotypes, were used for total RNA isolation. The sampling was done at 0, 2, 8, and 24 h after salt stress application [91 (link)]. RNA extraction was performed using the ZR Plant RNA MiniPrep™ Kit (Zymo Research, Irvine, CA, USA). DNA was eliminated from RNA samples by TURBO DNA-free™ Kit (Promega, Madison, WI, USA). The quality and quantity of the isolated RNAs were checked by agarose gel electrophoresis (1%) and spectrophotometrically using BioPhotometer (Eppendorf BioPhotometer plus, Hamburg, Germany).
The three replicates of each treatment for both genotypes were sequenced at the Beijing Genomics Institute (BGI, Shenzhen, China) using the Illumina NextSeq 500 platform as described in our previous publication [91 (link)].
The clean sequencing reads were submitted to SRA database at NCBI (accession number: PRJNA821484).
The three replicates of each treatment for both genotypes were sequenced at the Beijing Genomics Institute (BGI, Shenzhen, China) using the Illumina NextSeq 500 platform as described in our previous publication [91 (link)].
The clean sequencing reads were submitted to SRA database at NCBI (accession number: PRJNA821484).
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