Biomarker Expression Analysis in SCI

This study was reviewed and approved by the ethics committee of the Seventh Affiliated Hospital of Sun Yat-sen University and was adhered to the tenets of the Declaration of Helsinki. All participants provided written informed consent before they were included in the study. Blood samples were collected from 10 patients with SCI, 3 patients with closed noncentral nervous system trauma, and 3 healthy individuals. According to a method described in the literature, ACK Lysing Buffer (Thermo Fisher Scientific, Waltham, MA, USA) was used to lyse red blood cells to obtain white blood cells in blood (33 (link)). The RNA of these white cells was extracted using an RNA extraction kit (Beyotime, Shanghai, China). The RNA was reverse‐transcribed to cDNA using PrimeScript RT Master Mix (TAKARA, Dalian, China), and expression levels of the biomarkers of SCI and different ASI grades were verified by qPCR. PowerUp SYBR reagents (Thermo Fisher Scientific, Waltham, MA, USA) and the qPCR platform from Bio‐Rad (Hercules, CA, USA) were used. The primer sequences used for qPCR were listed in Table 1.