To examine the preventive effects of probiotics on anxiety/depression and colitis, mice were randomly assigned to six groups of seven mice each. First, test agents (C, vehicle [1% maltose]; NK33, 1 × 109 CFU/mouse/day of NK33; NK98, 1 × 109 CFU/mouse/day of NK33; Mix, 1 × 109 CFU/mouse/day of the (1:1) mixture of NK33 and NK98]; and PC, 1 mg/kg/day of buspirone) were orally gavaged or intraperitoneally injected into the mice daily for 5 days. Mice from the PC, NK33, NK98, Mix, and C groups were exposed to IS for 2 h from 24 h after the final treatment with test agents, as previously reported [28 (link)]. Normal control group (NC), not exposed to IS, was orally treated with 1% maltose in place of test agents. Behaviors and biochemical markers were assayed 20 h after the final IS treatment.
Probiotic Effects on Anxiety and Depression in Mice
To examine the preventive effects of probiotics on anxiety/depression and colitis, mice were randomly assigned to six groups of seven mice each. First, test agents (C, vehicle [1% maltose]; NK33, 1 × 109 CFU/mouse/day of NK33; NK98, 1 × 109 CFU/mouse/day of NK33; Mix, 1 × 109 CFU/mouse/day of the (1:1) mixture of NK33 and NK98]; and PC, 1 mg/kg/day of buspirone) were orally gavaged or intraperitoneally injected into the mice daily for 5 days. Mice from the PC, NK33, NK98, Mix, and C groups were exposed to IS for 2 h from 24 h after the final treatment with test agents, as previously reported [28 (link)]. Normal control group (NC), not exposed to IS, was orally treated with 1% maltose in place of test agents. Behaviors and biochemical markers were assayed 20 h after the final IS treatment.
Corresponding Organization : Kyung Hee University
Protocol cited in 4 other protocols
Variable analysis
- Exposure to IS (Inescapable Stress)
- Test agents: C (vehicle [1% maltose]), NK33 (1 × 10^9 CFU/mouse/day), NK98 (1 × 10^9 CFU/mouse/day), Mix (1 × 10^9 CFU/mouse/day of (1:1) mixture of NK33 and NK98), PC (1 mg/kg/day of buspirone)
- Behaviors
- Biochemical markers
- Normal control group (NC) not exposed to IS, treated with 1% maltose in place of test agents
- Positive control: PC group treated with 1 mg/kg/day of buspirone
- Negative control: C group treated with 1% maltose vehicle
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