Western blot analysis of combined adherent and floating cells was done as we have done before [6 (link)]. Antibodies used against H2AX, γH2AX (phospho-Ser139), PARP1, and actin were the same as previously described [6 (link)]. Also used were antibodies against Tankyrase (mouse monoclonal 19A449; IMGENEX), TRF1 (rabbit polyclonal ab1423; Abcam), and poly(ADP-ribose) chains (mouse monoclonal 3H2844; Santa Cruz).
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