Western Blot Analysis of Adherent and Floating Cells
Corresponding Organization : University of Nebraska Medical Center
Other organizations : University of Nebraska–Lincoln
Variable analysis
- None explicitly mentioned
- Protein expression levels of H2AX, γH2AX (phospho-Ser139), PARP1, Tankyrase, TRF1, and poly(ADP-ribose) chains
- Antibodies used against H2AX, γH2AX (phospho-Ser139), PARP1, and actin were the same as previously described [6 (link)]
- Also used were antibodies against Tankyrase (mouse monoclonal 19A449; IMGENEX), TRF1 (rabbit polyclonal ab1423; Abcam), and poly(ADP-ribose) chains (mouse monoclonal 3H2844; Santa Cruz)
- Positive controls: None explicitly mentioned
- Negative controls: None explicitly mentioned
Annotations
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