RNAs were extracted from whole blood (PAXgene tubes, Qiagen, Courtaboeuf, France) or PBMCs as previously isolated from EDTA tubes (Sigma Aldrich, Saint-Quentin Fallavier, France) using Ficoll (Eurobio, Lees Ulis, France) gradient and centrifugation as previously described [18 (link)] with RNeasy Mini Kit with a DNase I treatment to eliminate DNA contaminants and according to the manufacturer instructions (Qiagen, Courtaboeuf, France).
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