Histological and Morphometric Analysis of Metabolic Tissues

Samples of epididymal adipose tissue, liver, and pancreas were fixed with 10% formalin solution. After fixation, the specimens were dehydrated, embedded in paraffin, cut in a microtome to a thickness of 5 mm each, and stained with hematoxylin and eosin [54 ,55 ]. An expert pathologist performed the histological analysis of the liver and classified the samples according to a score system by Kleiner et al. [56 (link)]. A histological analysis of the pancreas followed the architecture of pancreas evaluation, according to changes in the islets of Langerhans [57 (link),58 (link)]. Images of the adipocyte area of the epididymal adipose tissue were taken using a Leica DFC 495 digital camera system (Leica Microsystems, Wetzlar, Germany) integrated into a Leica DM 5500B microscope (Leica Microsystems, Wetzlar, Germany) with a magnification of 20×. The images were analyzed using the Leica Application Suite software, version 4.0 (Leica Microsystems, Wetzlar, Germany), and the mean area of 100 adipocytes per sample was determined [14 (link)].

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Cardozo C.M., Inada A.C., Cardoso C.A., Filiú W.F., de Farias B.B., Alves F.M., Tatara M.B., Croda J.H., Guimarães R.D., Hiane P.A, & Freitas K.D. (2020). Effect of Supplementation with Hydroethanolic Extract of Campomanesia xanthocarpa (Berg.) Leaves and Two Isolated Substances from the Extract on Metabolic Parameters of Mice Fed a High-Fat Diet. Molecules, 25(11), 2693.

Publication 2020

Leica DFC 495 Leica DM 5500B microscope Leica Application Suite software, version 4.0

Adipocytes Adipose tissue Camera Embedded paraffin Eosin Epididymal Formalin Islets of langerhans Liver Microscope Microtome Pancreas Pathologist

Corresponding Organization : Universidade Federal de Mato Grosso do Sul

Other organizations : Universidade Estadual de Mato Grosso do Sul, Universidade Federal da Grande Dourados, Fundação Oswaldo Cruz

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Histological analysis of the liver classified according to a score system by Kleiner et al.
Histological analysis of the pancreas following the architecture of pancreas evaluation, according to changes in the islets of Langerhans
Mean area of 100 adipocytes per sample of epididymal adipose tissue

control variables

Samples of epididymal adipose tissue, liver, and pancreas were fixed with 10% formalin solution
Specimens were dehydrated, embedded in paraffin, cut in a microtome to a thickness of 5 mm each, and stained with hematoxylin and eosin

controls

No positive or negative controls were explicitly mentioned in the provided information.

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