Liver Cancer Tissue Profiling

Tissue from 34 patients with confirmed HCC undergoing resection at the Department of Surgery, University of Mainz, Germany were collected following patient informed consent and local ethics committee approval. This research has been approved by the ethic committee of the Landesärztekammer Rheinland-Pfalz on 05/15/13 (ethic code: 837.199.10 (7208). Detailed description of the cohort can be found [33 (link)]. Total RNA was extracted using the Qiagen RNEasy mini Kit (Qiagen GMBH, Hilden, Germany) following the manufacturer’s instructions. RNA quantity and purity were estimated using a Nanodrop ND-1000 Spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA), and integrity was assessed by Agilent 2100 Bioanalyzer (Agilent, Palo Alto, CA, USA). DNA was extracted using Qiagen Qiamp DNA Kit (Qiagen GMBH, Hilden, Germany) following the manufacturer’s instructions.