Impact of Chromobacterium violaceum on Mosquito Ovaries

Experiments were therefore performed in order to look at the impact of Chromobacterium violaceum on the development of ovaries within both infected inseminated females and uninfected mosquitoes. After the blood meals as above, inseminated females were anesthetized onto cold (− 20 °C) for five to ten minutes. Then we dissected individually their ovaries under a microscope as described in detailed in MR4 [22 (link)] (75 mosquitoes overall). The aspects of the ovaries were examined under microscope at 40 times magnification. Leica software (LAS-EZ-V3-3-0 for PC) was used to take pictures of the aspects of ovaries.