Protein extraction and Western blot analysis were performed according to the procedures described previously.16 (link) Briefly, the proteins of the cells on the membrane were incubated with a primary mouse monoclonal antibody against human RRM2 (1:1000 dilution; ab57653; Abcam) at 4 °C overnight. The mouse monoclonal antibody against human β-actin (1:5000 dilution; ab6276; Abcam) was used as a control.
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Protein Extraction and Western Blot Analysis
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Corresponding Organization : Shanghai Jiao Tong University
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Variable analysis
independent variables
- Primary mouse monoclonal antibody against human RRM2 (1:1000 dilution; ab57653; Abcam)
- Protein expression levels of human RRM2
- Mouse monoclonal antibody against human β-actin (1:5000 dilution; ab6276; Abcam)
- Positive control: Mouse monoclonal antibody against human β-actin (1:5000 dilution; ab6276; Abcam)
- Negative control: None specified
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