Multiplexed Analysis of Biomarkers

Mediators analysed in undiluted plasma (P), pericardial fluid (PF) and saliva (S) samples included IFN-gamma, IL-1alpha, IL-1beta, IL-6, IL-10, IL-12p40, TNF, CXCL8 (IL-8) and CXCL10 (IP-10), using customized Milliplex™ kits (HCYTOMAG-60 K, Millipore, St Charles, MO, USA) on the Bio-Plex platform (Bio-Rad Laboratories, Hercules, CA, USA) as described [8] (link). Caspases 3, 8 and 9 in undiluted pericardial fluid and plasma were measured using human in vitro ELISA kits from Abcam (Cambridge, UK), following the manufacturer's recommendations.

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Shenje J., Lai R.P., Ross I.L., Mayosi B.M., Wilkinson R.J., Ntsekhe M, & Wilkinson K.A. (2017). Effect of prednisolone on inflammatory markers in pericardial tuberculosis: A pilot study. International Journal of Cardiology. Heart & Vasculature, 18, 104-108.

Publication 2017

HCYTOMAG-60 K Bio-Plex platform

Caspases 3 Cxcl8 Elisa Gamma Human Il 10 Il 12p40 Il 1alpha Il 1beta Pericardial fluid Plasma Saliva

Corresponding Organization : Wellcome Centre for Infectious Diseases Research in Africa

Other organizations : Imperial College London

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Protocol cited in 1 other protocol

Variable analysis

independent variables

Undiluted plasma (P)
Pericardial fluid (PF)
Saliva (S)

dependent variables

IFN-gamma
IL-1alpha
IL-1beta
IL-10
IL-12p40
CXCL8 (IL-8)
CXCL10 (IP-10)
Caspases 3, 8 and 9

control variables

Not explicitly mentioned

positive controls

Not specified

negative controls

Not specified

Annotations

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