Glucose, glutamic acid and lactic acid were analyzed using the SBA-40D biosensor (Biology Institute, Shandong Academy of Sciences, Jinan, Shandong, China). Acetic acid, xylose, furfural, and HMF were analyzed on HPLC (LC-20AD, refractive index detector RID-10A, Shimadzu, Kyoto, Japan) with a Bio-Rad Aminex HPX-87H column (Bio-rad, Hercules, CA, USA) at 65 °C and 5 mM H2SO4 solution as the mobile phase at the flow rate of 0.6 mL/min. Phenolic compounds were analyzed on HPLC (UV/Vis detector SPD-20A, at 270 nm, Shimadzu, Kyoto, Japan) with a YMC-Pack ODS-A column (YMC Co., Kyoto, Japan) at 35 °C as mentioned before [13 (link)].
Cell growth was indicated by optical density at 600 nm (OD600) by the spectrophotometer Beckman Coulter DU800 (Beckman, Brea, CA, USA). The dry cell weight (DCW) was transformed 1 unit of OD600 to approximately 0.4 mg/mL of the dry cell weight.
Cell growth was indicated by optical density at 600 nm (OD600) by the spectrophotometer Beckman Coulter DU800 (Beckman, Brea, CA, USA). The dry cell weight (DCW) was transformed 1 unit of OD600 to approximately 0.4 mg/mL of the dry cell weight.
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