Culturing Human Colon Cancer Cell Lines

Human colon adenocarcinoma HCT-8 cells (Cat. No. CCL-244; ATCC, Manassas, VA) were cultured as previously described [26 (link),27 (link),72 ]. Human colon adenocarcinoma SW480 cells (Cat. No. CCL-228; ATCC, Manassas, VA) were cultured in L-15 Medium (Cat. No. 30–2008; ATCC) supplemented with 10% fetal bovine serum and Penicillin Streptomycin. Human colon carcinoma HCT-116 cells (Cat. No. CCL-247; ATCC, Manassas, VA) were cultured in McCoy’s 5a Medium (Cat. No. 30–2007; ATCC) supplemented with 10% fetal bovine serum and 1× antibiotic-antimycotic (Cat. No. 15240–062; Gibco). All cells were cultured at 37°C, with optimal humidity and 5% CO2.