Baicalein and Baicalin Induce Melanoma Apoptosis

Melanoma cells were cultured for 36 or 72 h in the presence of different concentrations of baicalein and baicalin, and apoptosis was analyzed after staining with PE-labeled Annexin V and 7-AAD (BD Biosciences, San Diego, CA, United States) (Liu et al., 2015 (link); Dou et al., 2018 (link); Wang et al., 2018 ). Stained cells were analyzed on a FACSCalibur (BD Bioscience) and the data were analyzed with the FlowJo software (Tree Star, Ashland, OR, United States).

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Huang L., Peng B., Nayak Y., Wang C., Si F., Liu X., Dou J., Xu H, & Peng G. (2020). Baicalein and Baicalin Promote Melanoma Apoptosis and Senescence via Metabolic Inhibition. Frontiers in Cell and Developmental Biology, 8, 836.

Publication 2020

PE-labeled Annexin V and 7-AAD FACSCalibur

7 aad Annexin v Apoptosis Baicalein Baicalin Cells Melanoma Tree

Corresponding Organization : Saint Louis University

Other organizations : China Pharmaceutical University

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Variable analysis

independent variables

Concentration of baicalein
Concentration of baicalin

dependent variables

Apoptosis

control variables

Culture duration (36 or 72 h)

controls

Positive control: Not specified
Negative control: Not specified

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