ECC-1 endometrial epithelial cells are an endometrial cancer cell line with characteristics of the endometrial luminal epithelial layer. They were obtained from the ATCC and their validity independently validated via Short Tandem Repeat (STR) DNA profiling of human cell lines per ATCC guidelines27 (link). They were routinely maintained in a 1:1 mix of DMEM:F12 Glutamax (Gibco, Invitrogen) supplemented with 1% P/S and 10% v/v fetal bovine serum (FBS, Gibco, Invitrogen). These cells were seeded and used for experimental purposes as described below.
L2-TSC (trophectodermal) cells (kind gift of Prof Susan Fisher, University of California, San Francisco) are derived from trophoblast stem cells23 (link). They were routinely maintained in a 1:1 mix of DMEM:F12 Glutamax (Gibco, Invitrogen) supplemented with 1% v/v P/S and 10% v/v FBS with addition of 10 ng/ml bovine fibroblast growth factor (bFGF) and 10 μM SB431542 (#1614, Tocris Bioscience). Cells were grown on flasks coated with 0.5% gelatin prior to experimental seeding.
L2-TSC (trophectodermal) cells (kind gift of Prof Susan Fisher, University of California, San Francisco) are derived from trophoblast stem cells23 (link). They were routinely maintained in a 1:1 mix of DMEM:F12 Glutamax (Gibco, Invitrogen) supplemented with 1% v/v P/S and 10% v/v FBS with addition of 10 ng/ml bovine fibroblast growth factor (bFGF) and 10 μM SB431542 (#1614, Tocris Bioscience). Cells were grown on flasks coated with 0.5% gelatin prior to experimental seeding.
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