VEGF-Aflox/flox mice were anaesthetized, and 1ul (10 ng) of either recombinant (r) VEGF-A (Prospec, Rehovot, Israel) or ultrapure DNase/RNase-free distilled water (vehicle) (Life Technologies, Carlsbad, CA, USA) were injected into the stromal layer of the cornea using a gas-powered microinjection system as described previously15 (link),28 (link). Immediately following the injection, the mice were inoculated with 200 PFUs of SC16 ICP0-Cre. On day 2 pi, the mice were injected again with r-VEGF-A or vehicle, and on day 7 pi, the mice were euthanized to harvest corneas for whole mount.
Protocol detail
VEGF-A Modulation of Corneal Immunity
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : University of Oklahoma Health Sciences Center
Top 5 similar protocols
Variable analysis
independent variables
- Injection of recombinant (r) VEGF-A
- Injection of ultrapure DNase/RNase-free distilled water (vehicle)
- Not explicitly mentioned
- VEGF-Aflox/flox mice
- Inoculation with 200 PFUs of SC16 ICP0-
Cre - Timing of injections (immediately following, day 2 post-inoculation)
- Positive control: Injection of recombinant (r) VEGF-A
- Negative control: Injection of ultrapure DNase/RNase-free distilled water (vehicle)
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!