Immunohistochemical and Immunofluorescence Analysis of DLBCL

Immunohistochemistry, as well as immunofluorescence on lymph node biopsies from DLBCL patients, were conducted as outlined previously [26 (link)]. The sections were stained using a primary antibody (anti-MECR, Proteintech, Cat. NO: 51027-2-AP; anti-RAN, Proteintech, Cat. NO: 67500-1-Ig; anti-ARSK, Bioss, Cat. NO: bs-9102R; anti-CD3, Proteintech, Cat. NO: 60181-1-Ig; anti-CD20, Proteintech, Cat. NO: 60271-1-Ig). The nucleus was stained using DAPI (Solarbio, Beijing, China) for use in immunofluorescence. Images of stained slides for these markers were scanned at 400× magnification using an optical microscope (Olympus Co., Tokyo, Japan). Immunohistochemistry results were quantified by counting the area of positive signals using Image J software. Fluorescent images were captured via a confocal laser microscopy system (Leica SP2).