Different human UCCs were used to represent the heterogeneity of UC: VM‐CUB1, RT‐112, UM‐UC‐3, J82, SW‐1710 and T24. UCCs were obtained from the DSMZ (Braunschweig, Germany) and Dr. H.B. Grossmann HB (Houston, USA). Two benign cell lines were also used as control: HBLAK, a spontaneously immortalized normal human uroepithelial cell line (Cellntec Advanced Cell Systems)
36 (link) and VHF2 dermal fibroblasts.
37 (link) UCC and VHF2 were cultivated in DMEM (Dulbecco's Modified Eagle Medium, 4.5 g/L D‐glucose, L‐glutamine, Gibco, Life Technologies Limited) supplemented with 10% heat inactivated fœtal bovine serum (Bio & Sell). HBLAK was cultivated in CnT Prime epithelial cell culture medium (Cellntec Advanced Cell Systems) and to detach the cells, accutase (Sigma‐Aldrich) was used.
Previously generated cisplatin‐resistant sublines J82 LTT, T24 LTT and RT‐112 LTT were maintained in cisplatin supplemented medium as recently described.
38 (link) Phase contrast images of quisinostat or DMSO treated cells were taken with the Nikon Eclipse microscope (Nikon) and the NIS elements software.
The identity of the cells is regularly verified by STR (short tandem repeat) analysis, and cells were tested for mycoplasma contamination by PCR.
36 (link) and VHF2 dermal fibroblasts.
37 (link) UCC and VHF2 were cultivated in DMEM (Dulbecco's Modified Eagle Medium, 4.5 g/L D‐glucose, L‐glutamine, Gibco, Life Technologies Limited) supplemented with 10% heat inactivated fœtal bovine serum (Bio & Sell). HBLAK was cultivated in CnT Prime epithelial cell culture medium (Cellntec Advanced Cell Systems) and to detach the cells, accutase (Sigma‐Aldrich) was used.
Previously generated cisplatin‐resistant sublines J82 LTT, T24 LTT and RT‐112 LTT were maintained in cisplatin supplemented medium as recently described.
38 (link) Phase contrast images of quisinostat or DMSO treated cells were taken with the Nikon Eclipse microscope (Nikon) and the NIS elements software.
The identity of the cells is regularly verified by STR (short tandem repeat) analysis, and cells were tested for mycoplasma contamination by PCR.
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