Investigating Biogenic Amine Production in Brine Microbiota

To investigate if the brine microbiota could lead to biogenic amine production, small-scale fermentation processes with an inoculum of 1% (vol/vol) brine 1 (brine sample B) were performed in sterile, closed 15 mL Falcon tubes (Sarstedt, Nümbrecht, Germany) at 15°C. Therefore, two different media were used. A liquid medium optimal for the production of biogenic amines was prepared as defined before (81 (link)) but without meat extract and supplemented with 5 g/L of NaCl. Also, a cheese medium as described above was used. Samples were taken after 0, 1, 2, and 4 weeks of fermentation, and biogenic amines were quantified as described previously (21 (link)).