Quantification of Insulin and Glucagon

Guinea pig anti-insulin (Dako A0564, 1:500), and rabbit anti-glucagon (Millipore AB932, 1:500) antibodies for immunofluorescence were used on paraffin-embedded pancreata fixed in 4% paraformaldehyde (PFA). Plasma glucose levels were obtained with the One Touch glucometer (Bayer). Plasma and total pancreatic insulin measurements were measured by radioimmunoassay or ELISA as described (Millipore, Crystal Chem)40 (link). Plasma leptin was measured by ELISA (Peprotech) and adiponectin by radioimmunoassay (Millipore).

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Rathjen T., Yan X., Kononenko N.L., Ku M.C., Song K., Ferrarese L., Tarallo V., Puchkov D., Kochlamazashvili G., Brachs S., Varela L., Szigeti-Buck K., Yi C.X., Schriever S.C., Tattikota S.G., Carlo A.S., Moroni M., Siemens J., Heuser A., van der Weyden L., Birkenfeld A.L., Niendorf T., Poulet J.F., Horvath T.L., Tschöp M.H., Heinig M., Trajkovski M., Haucke V, & Poy M.N. (2017). Regulation of body weight and energy homeostasis by neuronal Cell adhesion molecule 1. Nature neuroscience, 20(8), 1096-1103.

Publication 2017

A0564 AB932 adiponectin

Adiponectin Antibodies Elisa Embedded paraffin Glucagon Glucose Guinea pig Immunofluorescence Insulin Leptin Pancreata Pancreatic Paraformaldehyde Plasma Rabbit Radioimmunoassay Touch

Corresponding Organization :

Other organizations : Max Delbrück Center, Leibniz Association, University of Geneva, Charité - Universitätsmedizin Berlin, Yale University, Helmholtz Zentrum München, Heidelberg University, Wellcome Sanger Institute, Paul Langerhans Institute Dresden, TU Dresden

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Variable analysis

independent variables

Guinea pig anti-insulin (Dako A0564, 1:500) antibody
Rabbit anti-glucagon (Millipore AB932, 1:500) antibody

dependent variables

Plasma glucose levels
Plasma insulin measurements
Total pancreatic insulin measurements
Plasma leptin
Plasma adiponectin

control variables

Paraffin-embedded pancreata fixed in 4% paraformaldehyde (PFA)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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