Planaria, ≤6 mm in length, immobilized by either PC2-RNA interference (by dsRNA) (RNAi) [16 (link)]
or low percent ethanol (EtOH) [19 (link)]
See Steps 1–3
Planarian water, species appropriate [16 (link)]
SNARF-5F-AM (Molecular Probes S-23923, special packaging of 50 μg aliquots)
Keep at −20°C in the dark.
Pluronic F-127, 20% in Dimethyl Sulfoxide (DMSO) (Invitrogen P-3000MP)
Carbonyl cyanide 3-chlorophenylhydrazone (CCCP) protonophore (Sigma C2759)
Keep as 10 mM stock in DMSO at 4°C in dark.
2-(Cyclohexylamino)ethanesulfonic acid (CHES) buffer (Sigma C2885)
Sodium acetate (NaOAc) buffer (Sigma S2889)
or low percent ethanol (EtOH) [19 (link)]
See Steps 1–3
Planarian water, species appropriate [16 (link)]
SNARF-5F-AM (Molecular Probes S-23923, special packaging of 50 μg aliquots)
Keep at −20°C in the dark.
Pluronic F-127, 20% in Dimethyl Sulfoxide (DMSO) (Invitrogen P-3000MP)
Carbonyl cyanide 3-chlorophenylhydrazone (CCCP) protonophore (Sigma C2759)
Keep as 10 mM stock in DMSO at 4°C in dark.
2-(Cyclohexylamino)ethanesulfonic acid (CHES) buffer (Sigma C2885)
Sodium acetate (NaOAc) buffer (Sigma S2889)
