Human ovarian tumor cells (HeyA8) were a gift from Dr. Anil Sood, MD Anderson Cancer Center [12 (link); 18 (link)]. Tumor cells were maintained in 5% CO2 at 37 °C in RPMI-1640 media plus 15% fetal bovine serum and 0.1 % antibiotics. Tumor cell viability was measured by an enzymatic MTT (3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide) assay. HeyA8 cells (3,000 cells /well) were seeded in flat bottom 96 well plates. After 24 hours fresh media was added to the wells +/− PFT-μ (5–20 μM) and +/− paclitaxel (15 nM) for 72 hours. After 72 hrs. MTT (0.15%) was added to each well and incubated for 2 hrs. followed by DMSO. Colorimetric changes were measured at 570 nm on a Biotek Epoch plate reader.
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Assessing Ovarian Tumor Cell Viability
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Antibiotics
Assay
Bromide
Cancer
Cell viability
Cells
Colorimetric
Dmso
Enzymatic
Epoch
Fetal bovine serum
Human
Ovarian tumor
Paclitaxel
Tumor
Corresponding Organization : The University of Texas MD Anderson Cancer Center
Other organizations : University Medical Center Utrecht
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Variable analysis
independent variables
- PFT-μ (5–20 μM)
- Paclitaxel (15 nM)
- Tumor cell viability measured by an enzymatic MTT (3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide) assay
- HeyA8 cells (3,000 cells /well) were seeded in flat bottom 96 well plates
- Cells were maintained in 5% CO2 at 37 °C in RPMI-1640 media plus 15% fetal bovine serum and 0.1 % antibiotics
- After 24 hours, fresh media was added to the wells
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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