The experimental materials and their sources
are as follows. Adenosine
monophosphate (AMP), dithiothreitol (DTT), dimethyl sulfoxide (DMSO),
ethylenediaminetetraacetic acid (EDTA), 1-hydroxypyrene (1-HP), 4-hydroxytamoxifen
(afimoxifene, TAM), imidazole, isopropyl thio-β-d -galactopyranoside
(IPTG), Luria broth (LB), lysozyme, β-mercaptoethanol (β-ME),
pepstatin A, Na2HPO4, and NaH2PO4 were obtained from Sigma. Ampicillin, HEPES, KCl, KOH, MgCl2, NaCl, and phenylmethanesulfonyl fluoride (PMSF) were purchased
from Fisher Scientific. Glutathione- and nickel-chelating resins were
obtained from GE Healthcare. Competent Escherichia coli [BL21(DE3)] cells were purchased from Agilent Technologies. PAP,
PAPS, and [35S]PAPS were synthesized in house as previously
described12 (link),16 (link),17 (link) and were ≥98%
pure as assessed by anion-exchange high-performance liquid chromatography.
are as follows. Adenosine
monophosphate (AMP), dithiothreitol (DTT), dimethyl sulfoxide (DMSO),
ethylenediaminetetraacetic acid (EDTA), 1-hydroxypyrene (1-HP), 4-hydroxytamoxifen
(afimoxifene, TAM), imidazole, isopropyl thio-β-
(IPTG), Luria broth (LB), lysozyme, β-mercaptoethanol (β-ME),
pepstatin A, Na2HPO4, and NaH2PO4 were obtained from Sigma. Ampicillin, HEPES, KCl, KOH, MgCl2, NaCl, and phenylmethanesulfonyl fluoride (PMSF) were purchased
from Fisher Scientific. Glutathione- and nickel-chelating resins were
obtained from GE Healthcare. Competent Escherichia coli [BL21(DE3)] cells were purchased from Agilent Technologies. PAP,
PAPS, and [35S]PAPS were synthesized in house as previously
described12 (link),16 (link),17 (link) and were ≥98%
pure as assessed by anion-exchange high-performance liquid chromatography.
