Protocol detail

Quantitative RT-PCR Analysis of Gene Expression

Find Similar Protocols

Total mRNA was isolated from colon or indicated cell type using the Omega Total RNA Kit according to the manufacturer’s protocol. cDNA was generated using the RNA to cDNA Ecodry Premix Kit (Clontech) according to the manufacturer’s protocol. cDNA was used as a template for quantitative real-time PCR using SYBR Green Master Mix (Roche), and gene-specific primers (18 (link)). PCR analysis was performed using a MyiQ5 ICycler (BioRad). Gene expression was normalized relative to Gapdh.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Swafford D., Shanmugam A., Ranganathan P., Hussein M.S., Koni P.A., Prasad P.D., Thangaraju M, & Manicassamy S. (2018). Canonical Wnt Signaling in CD11c+ Antigen Presenting Cells Regulates Microbiota-Induced Inflammation and Immune Cell Homeostasis in the Colon. Journal of immunology (Baltimore, Md. : 1950), 200(9), 3259-3268.

Publication 2018

Ecodry Premix Kit SYBR Green Master Mix MyiQ5 ICycler

Cdna Cell type Colon Gapdh Gene Gene expression Mrna Primers Quantitative real time pcr Sybr green

Top 5 similar protocols

Variable analysis

independent variables

Cell type (colon or indicated cell type)

dependent variables

Gene expression

control variables

Gapdh (used for normalization)

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!