Quantitative Analysis of Apoptosis Regulators

Analysis of Bax, Bcl-2 and caspase-3 protein was performed using the ELISA method. The MCF-7 cells (2 × 10⁴/well) were seeded into 96-well plate. The cells were treated with free AZA, AZA-LPO and blank liposome samples for 24 h. Thereafter, the wells were incubated with monoclonal antibodies against Bax (Ab-1) (DRG^® Human Bax ELISA Kit), Bcl-2 (Zymed^® Bcl-2 ELISA Kit) and caspase-3 (Invitrogen ELISA kit). The procedure was followed as per manufacturer instruction [49 (link)].

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Kesharwani P., Md S., Alhakamy N.A., Hosny K.M, & Haque A. (2021). QbD Enabled Azacitidine Loaded Liposomal Nanoformulation and Its In Vitro Evaluation. Polymers, 13(2), 250.

Publication 2021

Bcl-2 ELISA Kit

Bcl 2 Caspase 3 Cells Elisa Human Liposome Mcf 7 cells Monoclonal antibodies Protein

Corresponding Organization : Jamia Hamdard

Other organizations : King Abdulaziz University, Prince Sattam Bin Abdulaziz University

Top 5 similar protocols

Variable analysis

independent variables

Free AZA
AZA-LPO
Blank liposome samples

dependent variables

Bax protein
Bcl-2 protein
Caspase-3 protein

control variables

MCF-7 cells (2 × 10^4/well)
96-well plate
Incubation time (24 h)

controls

Positive control: Not explicitly mentioned
Negative control: Blank liposome samples

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