D-004, GSE, VE and tamsulosin were suspended in Tween-65/H2O vehicle (2%). All treatments (including the vehicle) were given as single oral doses by gastric gavage (5 mL/kg of body wt) one hour before inducing PHE-urodynamic impairment.
PHE was diluted in saline solution (5 mg/mL) and administered by subcutaneous injection (s.c).
Rats were randomized into eight groups (ten rats/group): a negative vehicle control and seven groups treated with PHE: a positive control, three treated with D-004 (200, 400 and 800 mg/kg), three with tamsulosin (0.4 mg/kg), one with GSE (250 mg/kg) and one with VE (250 mg/kg).
After treatment completion, rats were weighed, then anesthetized under ether atmosphere and sacrificed by complete bleeding from the abdominal aorta. Prostate was immediately separated from bladder and both of them were removed and weighed in analytical balance Mettler Toledo.
PHE was diluted in saline solution (5 mg/mL) and administered by subcutaneous injection (s.c).
Rats were randomized into eight groups (ten rats/group): a negative vehicle control and seven groups treated with PHE: a positive control, three treated with D-004 (200, 400 and 800 mg/kg), three with tamsulosin (0.4 mg/kg), one with GSE (250 mg/kg) and one with VE (250 mg/kg).
After treatment completion, rats were weighed, then anesthetized under ether atmosphere and sacrificed by complete bleeding from the abdominal aorta. Prostate was immediately separated from bladder and both of them were removed and weighed in analytical balance Mettler Toledo.
