Membrane Current Measurement Protocols

Macroscopic membrane
current was recorded 48–72 h after injection as described previously.^{8 (link)} The tested compounds were applied at pH 5.5 at
various concentrations when the inward current reaches maximum. The
compounds were applied for 2 min, and residual membrane current was
compared with the membrane current before the application of compounds.
Membrane currents were analyzed with pCLAMP 10.0 software package
(Axon Instruments, Sunnyvale, CA).

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Rey-Carrizo M., Barniol-Xicota M., Ma C., Frigolé-Vivas M., Torres E., Naesens L., Llabrés S., Juárez-Jiménez J., Luque F.J., DeGrado W.F., Lamb R.A., Pinto L.H, & Vázquez S. (2014). Easily Accessible Polycyclic Amines that Inhibit the Wild-Type and Amantadine-Resistant Mutants of the M2 Channel of Influenza A Virus. Journal of Medicinal Chemistry, 57(13), 5738-5747.

Publication 2014

pCLAMP 10.0 software package

Axon Membrane

Corresponding Organization :

Other organizations : Universitat de Barcelona, Northwestern University, KU Leuven, University of California, San Francisco

Top 5 similar protocols

Variable analysis

independent variables

Tested compounds

dependent variables

Macroscopic membrane current
Residual membrane current

control variables

PH 5.5
Membrane currents analyzed with pCLAMP 10.0 software package

controls

Membrane current before the application of compounds

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