RNA was isolated from cells in triplicate wells treated with ethanol (vehicle) or estradiol (1nM) for 24 hours using TriReagent (Roche). Data shown is representative of two to nine replicates of each qPCR experiment which was performed as previously described (12 (link)). Student’s T-tests were performed to determine statistical significance between treatment groups in each experiment.
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Ethanol and Estradiol Treated Cells
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Corresponding Organization :
Other organizations : University of Minnesota, Twin Cities Orthopedics, Mayo Clinic, The University of Texas Southwestern Medical Center
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Variable analysis
independent variables
- Ethanol (vehicle)
- Estradiol (1nM)
- Gene expression measured by qPCR
- RNA isolated from cells in triplicate wells
- Cells treated for 24 hours
- TriReagent (Roche) used for RNA isolation
- Positive control: Not specified
- Negative control: Ethanol (vehicle)
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