MAPseq with Modified Sindbis Virus

Modified sindbis virus for MAPseq was obtained from the MAPseq Core Facility at Cold Spring Harbor Laboratory^{7 (link)}. The viral library used in this study had a diversity of 20,000,000 unique barcodes. Retro-AAV2 coding for mCherry (pAAV2-hSyn-mCherry, Addgene #114472, 2 × 10¹³ GC/ml) and green fluorescent protein (pAAV2-hSyn-eGFP, Addgene #50465, 2.2 × 10¹³ GC/ml) under the human synapsin promoter were obtained from Addgene. All viruses were stored at −80°C and aliquots were thawed over wet ice immediately prior to injection.

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Zeisler Z.R., London L., Janssen W.G., Fredericks J.M., Elorette C., Fujimoto A., Zhan H., Russ B.E., Clem R.L., Hof P.R., Stoll F.M, & Rudebeck P.H. (2023). High-throughput sequencing of macaque basolateral amygdala projections reveals dissociable connectional motifs with frontal cortex. bioRxiv.

Publication Preprint 2023

pAAV2-hSyn-eGFP

Cold Green fluorescent protein Human Library Sindbis virus Synapsin Viruses

Corresponding Organization : Icahn School of Medicine at Mount Sinai

Other organizations : Cold Spring Harbor Laboratory, NYU Langone Health, Nathan Kline Institute for Psychiatric Research

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Variable analysis

independent variables

Modified sindbis virus for MAPseq

dependent variables

Not explicitly mentioned

control variables

Retro-AAV2 coding for mCherry (pAAV2-hSyn-mCherry, Addgene #114472, 2 × 10^13 GC/ml)
Retro-AAV2 coding for green fluorescent protein (pAAV2-hSyn-eGFP, Addgene #50465, 2.2 × 10^13 GC/ml)

controls

Positive control: Retro-AAV2 coding for mCherry and green fluorescent protein
Negative control: Not explicitly mentioned

Annotations

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