Letrozole Administration in Pregnant Rats

In this experimental study, a total of 20 female Sprague-Dawley rats (130-180 gr, 8 wk) were obtained from the Center of Comparative and Experimental Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Female rats were maintained under standard conditions (12 hr light/dark cycle, temperature of 23

\pm

3 C, and relative humidity of 25

\pm

5%) with free access to rodent food and water. After adaptation to the new environment, 18 female rats in the proestrous or estrous phase were joined with sexually experienced adult male rats (mean weight: 375 gr) with a 2:1 female/male ratio overnight. In the morning after mating, a vaginal plug or spermatozoa in the vaginal smear was considered as the first day of pregnancy (GD1). 15 pregnant rats were randomly selected for letrozole administration during pregnancy.

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Shaaban Z., Derakhshanfar A., Reza Jafarzadeh Shirazi M., Javad Zamiri M., Moayedi J., Vahedi M, & Valizadeh A. (2023). The effects of letrozole-induced maternal hyperandrogenism on sexual behaviors, testicular histology, and serum biochemical traits in male offspring rats: An experimental study. International Journal of Reproductive Biomedicine, 21(1), 71-82.

Publication 2023

Adaptation Adult Estrous Female Food Humidity Letrozole Male Pregnancy Rats Rodent Spermatozoa Sprague dawley rats Vaginal Vaginal smear

Corresponding Organization :

Other organizations : Shiraz University, Shiraz University of Medical Sciences

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Variable analysis

independent variables

Letrozole administration during pregnancy

dependent variables

Not explicitly mentioned

control variables

Female Sprague-Dawley rats (130-180 gr, 8 wk)
Standard conditions (12 hr light/dark cycle, temperature of 23 ± 3 C, and relative humidity of 25 ± 5%) with free access to rodent food and water
Adult male rats (mean weight: 375 gr) with a 2:1 female/male ratio for mating

controls

Positive control: Pregnant rats with mating (presence of vaginal plug or spermatozoa in the vaginal smear)
Negative control: Not mentioned

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