Quantifying Chromosome Pairing Dynamics

Leptotene/zygotene nuclei staining positively for S8 pSUN-1 were randomly selected from high-resolution Z-stacks. The region of the inner nuclear envelope where chromosomes cluster during pairing (i.e., the region corresponding to adjacent or overlapping DAPI and NPC staining) was selected as a region of interest (ROI). A line-plot of raw pixel intensities for S8 pSUN-1 and GRAS-1::GFP signals along the ROI was generated using the Multichannel Plot Profile plugin for Fiji ImageJ [70 (link)]. Pixel intensity values for each ROI were used to calculate the Pearson correlation coefficient as a measure of normalized covariance of the signals. Coefficients calculated from 45 leptotene/zygotene nuclei across 9 gonads were then averaged.

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Martinez-Garcia M., Naharro P.R., Skinner M.W., Baran K.A., Lascarez-Lagunas L.I., Nadarajan S., Shin N., Silva-García C.G., Saito T.T., Beese-Sims S., Diaz-Pacheco B.N., Berson E., Castañer A.B., Pacheco S., Martinez-Perez E., Jordan P.W, & Colaiácovo M.P. (2023). GRAS-1 is a novel regulator of early meiotic chromosome dynamics in C. elegans. PLOS Genetics, 19(2), e1010666.

Publication 2023

Chromosomes Dapi Gonads Gras Nuclear envelope Nuclei

Corresponding Organization : Boston VA Research Institute

Other organizations : Johns Hopkins University, Uniformed Services University of the Health Sciences, MRC London Institute of Medical Sciences

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Variable analysis

independent variables

Region of the inner nuclear envelope where chromosomes cluster during pairing (i.e., the region corresponding to adjacent or overlapping DAPI and NPC staining)

dependent variables

Pearson correlation coefficient as a measure of normalized covariance of the S8 pSUN-1 and GRAS-1::GFP signals

control variables

Leptotene/zygotene nuclei staining positively for S8 pSUN-1

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