Quantifying Myocardial Angiogenesis via CD34

Myocardial tissue sections were stained with anti-CD34 primary antibody (1 : 200, Abcam, Cambridge, UK). Vascular endothelial cells were immunohistochemically labeled with CD34 to calculate microvessel density (MVD) [21 (link)], which was quantified by counting the number of brown endothelial cells or endothelial cell clusters. Five microscopic fields were randomly selected for each tissue section under 400× magnification, and their average values were taken as the MVD of each sample.

Free full text: Click here

Sawuer G., Ma X.K., Zhang Y.J., Zhang X.M., Ainiwaer Z, & An D.Q. (2021). Tianxiangdan Improves Coronary Microvascular Dysfunction in Rats by Inhibiting Microvascular Inflammation via Nrf2 Activation. Evidence-based Complementary and Alternative Medicine : eCAM, 2021, 4114784.

Publication 2021

anti-CD34 primary antibody

Anti antibody Endothelial Endothelial cell Microscopic Myocardial tissue Tissue Vascular endothelial cells

Corresponding Organization : Xinjiang Medical University

Other organizations : First Affiliated Hospital of Shihezi University Medical College

Top 5 similar protocols

Variable analysis

independent variables

Anti-CD34 primary antibody (1 : 200, Abcam, Cambridge, UK)

dependent variables

Microvessel density (MVD)

control variables

Microscopic fields (five random fields per tissue section)

controls

No positive or negative controls were explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!