Animal procedures were conducted in accordance with standards from the National Institutes of Health and under the approval of the Princeton University Institutional Animal Care and Use Committee.
Experimental animals for imaging were comprised of 20 mice (11 males and 9 females) cross bred between DAT::cre (Jackson Laboratory strain 006660; (Lammel et al. 2015 (link))) and the Ai148 GCaMP6f reporter line (Jackson Laboratory strain 030328; (Daigle et al. 2018 (link))), a cross extensively characterized previously (Engelhard et al. 2019 (link)). The above mice were housed with littermates until 2 weeks prior to imaging, at which time they were singly housed with enrichment materials (running wheel, nestlets). Stimulus mice and mice used for snRNA-sequencing were C57 / BL6 WT (Jackson Laboratory) males and females between the ages of 10 and 16 weeks. Stimulus mice were non-littermates of imaged animals. Stimulus animals were housed with their cage mates throughout the experiment. Food and water were given ad libitum, except during 2 experimental food deprivation days for both imaging mice and hunger state manipulated sequencing mice. Prior to and throughout experimental assays, experimental and stimulus animals were housed under a 12 H light-dark cycle with experiments exclusively taking place during the dark phase.
Experimental animals for imaging were comprised of 20 mice (11 males and 9 females) cross bred between DAT::cre (Jackson Laboratory strain 006660; (Lammel et al. 2015 (link))) and the Ai148 GCaMP6f reporter line (Jackson Laboratory strain 030328; (Daigle et al. 2018 (link))), a cross extensively characterized previously (Engelhard et al. 2019 (link)). The above mice were housed with littermates until 2 weeks prior to imaging, at which time they were singly housed with enrichment materials (running wheel, nestlets). Stimulus mice and mice used for snRNA-sequencing were C57 / BL6 WT (Jackson Laboratory) males and females between the ages of 10 and 16 weeks. Stimulus mice were non-littermates of imaged animals. Stimulus animals were housed with their cage mates throughout the experiment. Food and water were given ad libitum, except during 2 experimental food deprivation days for both imaging mice and hunger state manipulated sequencing mice. Prior to and throughout experimental assays, experimental and stimulus animals were housed under a 12 H light-dark cycle with experiments exclusively taking place during the dark phase.
