Western blot analysis was carried out as previously described (Tomimaru et al., 2013a (link)). In brief, immunoblots were probed with primary antibodies generated against β-catenin, β-actin (Cell Signaling Technology, Beverly, MA), cyclin D1, and PCNA (Santa Cruz Biotechnology, Inc., Santa Cruz, CA) and detected with HRP-labeled anti-mouse or anti-rabbit IgG (Amersham Pharmacia Biotech, Buckinghamshire, UK) using SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Scientific, Rockford, IL). Positive signals were captured by the Image analyzer LAS-1000 plus (Fujifilm, Tokyo, Japan) and the band intensity of protein was determined using Image Gauge version 3.45 (Fujifilm).
Protocol detail
Western Blot Analysis of Signaling Proteins
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
β-catenin
β-actin
cyclin D1
PCNA
SuperSignal West Femto Maximum Sensitivity Substrate
Image Gauge version 3.45
Actin
Anti igg
Antibodies
Cyclin d1
Immunoblots
Mouse
Pcna
Protein
Rabbit
Sensitivity
Western blot analysis
β catenin
Corresponding Organization : Rhode Island Hospital
Top 5 similar protocols
Variable analysis
independent variables
- Not explicitly mentioned
- Levels of β-catenin
- Levels of cyclin D1
- Levels of PCNA
- Levels of β-actin
- Positive control: Not mentioned
- Negative control: Not mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!